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Fig. 1. Identification of Xwnt11 as a target of Xbra. (A) Induction of
Xwnt11 by Xbra-GR. Animal caps derived from embryos injected
with 50 pg Xbra-GR RNA or left uninjected were dissected at
blastula stages and then treated with 10-6 M dexamethasone (DEX)
for 3 hours or left untreated. RNA was extracted and analysed by
northern blotting. ODC and Xbra served as loading and positive
controls, respectively. Note that DEX alone does not induce
expression of Xwnt11. (B-G) Comparison of expression patterns of
Xwnt11 (B-D) and Xbra (E-G) at stage 11 (B,E), stage 12.5 (C,F) or
stage 14 (D,G). |