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tbxtxenopus   

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Experiment details for tbxt

Xwnt11 is a target of Xenopus Brachyury: regulation of gastrulation movements via Dishevelled, but not through the canonical ...

Xwnt11 is a target of Xenopus Brachyury: regulation of gastrulation movements via Dishevelled, but not through the canonical Wnt pathway.

Gene Clone Species Stages Anatomy
tbxt.S laevis NF stage 11 marginal zone , circumblastoporal collar
tbxt.S laevis NF stage 14 mesoderm , marginal zone , circumblastoporal collar , anterior neural fold

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  Fig. 1. Identification of Xwnt11 as a target of Xbra. (A) Induction of Xwnt11 by Xbra-GR. Animal caps derived from embryos injected with 50 pg Xbra-GR RNA or left uninjected were dissected at blastula stages and then treated with 10-6 M dexamethasone (DEX) for 3 hours or left untreated. RNA was extracted and analysed by northern blotting. ODC and Xbra served as loading and positive controls, respectively. Note that DEX alone does not induce expression of Xwnt11. (B-G) Comparison of expression patterns of Xwnt11 (B-D) and Xbra (E-G) at stage 11 (B,E), stage 12.5 (C,F) or stage 14 (D,G).