| |
Fig. 4. Xfz7 changes the pattern of chordin and Xbra. Albino embryos at the four-cell stage were injected in the marginal region of all blastomeres with 0.5 ng of Xfz7 mRNA. (A,C,E) Expression of chd in uninjected and (B,D,F) Xfz7-injected embryos was examined at stage 10.5, 12 and 18 by whole-mount in situ hybridization. The early pattern of chd is unchanged in injected embryos (A,B). Toward the end of gastrulation, chd-positive cells do not converge to the dorsal midline (C,D) and stay at the site of the closing blastopore (E,F), indicated by arrows. (G,I,K) Expression of Xbra in uninjected and (H,J,L) Xfz7-injected embryos was examined at stage 10.5, 12 and 18. (L) Small inserted picture. Note the ectopic Xbra expression in Xfz7-injected embryos on ventral and lateral sides (arrows). |
