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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
tbxt	xenopus

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Experiment details for tbxt

Cloning and spatiotemporal expression of RIC-8 in Xenopus embryogenesis.

Cloning and spatiotemporal expression of RIC-8 in Xenopus embryogenesis.

Gene	Clone	Species	Stages	Anatomy
tbxt		tropicalis	NF stage 10	marginal zone , marginal zone (sensu neural)
tbxt		tropicalis	NF stage 15	neuroectoderm , neural groove , anterior neural fold , neural tube

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Fig.3. Comparative analysis of XtRIC-8 and control markers by whole mount in situ hybridization at early developmental stages of Xenopus tropicalis embryos. (A) The tissue specific expression pattern of ric-8 at different early stages of X. laevis and X. tropicalis embryonic development was analyzed by whole mount in situ hybridization. Probes are indicated in each panel at the left, stages are indicated at the top of each panel. XtRIC-8 antisense probe is shown on panels f–j, whereas XlRIC-8 probe is shown on panels from k to o. XtRIC-8 sense probe was used as negative control (panels a–e). Animal views are shown for embryos at stage 3, 6 and 8; vegetal views for embryos at stage 10 and dorsal views for embryos at stage 15 and 19. Animal–Vegetal (A–V) lateral view is shown in the inset at stages 3 and 6 (panels f and g). (B) The tissue specific expression of different control markers was determined at different developmental stages of X. tropicalis embryos by whole mount in situ hybridization. Sox-2, N-tubulin, EphA4, Twist, Slug and Xbra were used as control markers. Probes are indicated at the left side of each row and stages at the bottom-left side of each panel. Vegetal views are shown for stage 10; dorsal views for stage 15 and 19. Red arrows indicate specific structures that were labeled as: r (rhombomeres), nt (neural tube), nc (neural crest), mz (marginal zone), bl (blastopore lip), ppe (pre-placodal ectoderm) and np (neural plate).