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Fig. 2. Effect of Xdelta1-MO on gastrulation and Xbra expression. Xdelta1-MO and tracer mRNAs were in- jected into the lateral side of one blastomere at the 2-cell stage. Gastrulation (A-C) and Xbra expression (D-F) were examined in the injected embryo at st.12 and st.10.5, respectively. All embryos are shown in vegetal view with the ventral side up, and arrows indicate the injection side. GFP (A-C) and -gal (D-F) were used as tracers of the injection side. In contrast to the normal gastrulation of the GFP-injected control embryo (A), Xdelta1-MO caused the gastrulation defect in the injection side (B), which was completely rescued by co-injection of NICD (C). Xdetla1- MO inhibited Xbra expression (E), which was rescued by co-injection of NICD (F). Injection of -gal into control embryos showed no effect on Xbra expression (D). |
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Fig. 7. (Right) Effect of XESR1 and XESR5 on the gene expression of Xbra. Synthesized RNAs of XESR1 (B), DN-XESR1 (C), XESR5 (D) or DN- XESR5 (E) were injected into the lateral side of one blastomere at the 2-cell stage together with -gal as a lineage tracer, and gene expression of Xbra was examined at st.10.5 by whole-mount in situ hybridization. Control embryo was injected with -gal alone (A). All embryos are shown in vegetal view with the ventral side up. Arrows indicate the injection side. |
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Fig. 1. Comparison of gene expression profiles of Xbra and Xdelta1. (A-D) Gene expression of Xbra (A,B) and Xdelta1 (C,D) at st.9 (A,C) and st.11.5 (B,D). All embryos are shown in lateral view with the dorsal side right. Xdelta1 shows the zygotic expression prior to Xbra expression. (E,F) Double in situ hybridization of Xbra (blue) and Xdelta1 (red) in the st.11.5 embryo. (E) Vegetal view with the dorsal side up. (F) Dorsal view with the animal side up. Gene expression of Xbra and Xdelta1 overlapped with each other in the same marginal zone. Xdelta1 expression was recognized in the Xbra-free dorsal area (arrowheads). |
