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Experiment details for tbxt

Wacker SA et al. (2004) Assay

Timed interactions between the Hox expressing non-organiser mesoderm and the Spemann organiser generate positional information during vertebrate gastrulation.

Gene Clone Species Stages Anatomy
tbxt.S laevis NF stage 10.5 to NF stage 11 marginal zone , dorsal marginal zone , involuting marginal zone , involuted dorsal mesoderm , upper blastopore lip

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  Fig. 1. Spatial and temporal Hox expression during gastrulation. Results for Hoxd-1, Hoxb-4, Hoxc-6, Hoxa-7 and Hoxb-9 are shown, Hoxa-1, Hoxb-1 and Hoxb-7 also fit this sequence (not shown), Hoxd-13 expression did not begin before the end of gastrulation (stage 15, not shown). (A) WISH for five Hox genes at five different stages. Vegetal views, dorsal up. (B) Diagram showing the onset of the temporally colinear expression of five different Hox genes as analysed with WISH (red) and PCR (blue). (C) Localisation of Hoxd-1 expression in mesoderm and ectoderm. Embryos cut into halves across the dorsolateral blastopore lip at stages 10.5 and 11. One half analysed with Xbra, the other with Hoxd-1. The Xbra expression domain is outlined. The initial Hoxd-1 expression is located within the mesoderm. At stage 11, it is expanded into the presumptive neurectoderm (arrowheads).