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Fig. 1. Spatial and temporal Hox expression during gastrulation. Results for Hoxd-1, Hoxb-4, Hoxc-6, Hoxa-7 and Hoxb-9 are shown, Hoxa-1, Hoxb-1 and
Hoxb-7 also fit this sequence (not shown), Hoxd-13 expression did not begin before the end of gastrulation (stage 15, not shown). (A) WISH for five Hox genes
at five different stages. Vegetal views, dorsal up. (B) Diagram showing the onset of the temporally colinear expression of five different Hox genes as analysed
with WISH (red) and PCR (blue). (C) Localisation of Hoxd-1 expression in mesoderm and ectoderm. Embryos cut into halves across the dorsolateral blastopore
lip at stages 10.5 and 11. One half analysed with Xbra, the other with Hoxd-1. The Xbra expression domain is outlined. The initial Hoxd-1 expression is located
within the mesoderm. At stage 11, it is expanded into the presumptive neurectoderm (arrowheads). |