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Fig. 6. Geminin suppression of mesoderm and endoderm depends on Polycomb but is independent of Oct25/60 and Sox2 activities. Geminin RNA (120 pg) with or without 8 ng of MOs (16 ng Oct60MO) + 30 pg β-galactosidase RNA was injected into one cell of eight-cell embryos, with in situ hybridization at stage 11.5. Red-gal staining and white or red arrowheads mark injected regions. Below are additive results for four experiments shown as fractions of embryos with indicated effects (embryo numbers analyzed are in parentheses). |
