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Fig. 1. Overexpression of Klf4 in Xenopus early embryos. (A,B) The effect of Klf4 mRNA injection on gastrulation (A) and on body axis formation (B). (C) Quantification of phenotypes shown in A and B in triplicate. (D-F) The influence of Klf4 mRNA injection on mesoderm (D), endoderm (E) and ectoderm (F). Embryos in D were placed in vegetal views; those in E and F were placed in vegetal view (v) and animal view (a), separately. (G) Quantification of embryos with gene expression observed in D,E,F. In these experiments, 400 pg of Klf4 mRNA were injected into the equatorial region of all blastomeres of two-cell or four-cell embryos. (H,I) lacZ labeling of targeted injection into one animal-ventral blastomere at the eight-cell stage and whole-mount in situ hybridization detection of Sox17-alpha (H) and Sox2 (I) expression. Embryos were also placed in animal (a) and vegetal (v) views, respectively, as indicated at the top of the panels. (J) Quantification of embryos with normal or ectopic gene expression in H and I. lacZ mRNA was injected at 20 pg/nl; Klf4 mRNA was injected at 40 pg/nl. The arrows indicate the blastopore (bl). In all the panels, dorsal is up for Sox2-stained embryos. |
