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Fig3. 32-cell sizzled morphant phenotypes and rescue.
Sizzled morpholino was injected, together with GFP RNA, into the C2/C3 blastomeres of 32 cell stage embryos. Embryos were sorted based on fluorescence at stage 10. Stage 11 embryos were stained for xbra RNA ((A)-control, (B,C)-morphant); stage 18 embryos were stained for snail2 ((D)-control, (E,F)-morphant), twist1 ((G)-control, (H,I)-morphant), sox9 ((J)-control, (K,L)-morphant) RNAs. Effects on myoD RNA were examined at stage 25 ((M)-control, (N)-morphant). (O) sox9 expression in sizzled MO embryos was rescued by sizzled RNA injection (200 pgs/embryo) but not by sizzled D92N RNA (green bars indicate percentage of embryos with a wild type phenotype, red bars indicate loss of sox9 RNA staining).
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