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Supplementary Fig. 3. Knockdown of ADAM13 has no apparent influence on xbra expression. One-cell stage embryos were injected with the indicated MO (12 ng each), and cultured to stage ~11. In situ hybridization was carried out for xbra, and multiple embryos of the same injection groups are shown in A and C. Embryos were also sectioned as illustrated in E (with animal cap removed to reveal the staining of xbra in mesoderm), and one representative example of each group is shown in B and D (animal pole views with dorsal at the top). |
