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Fig. 5. ASCL1-NT is necessary and sufficient for inhibiting mesendoderm induction. (A) Schematic depiction of serial-deletion mutants of ASCL1. (B) Vegetal view of representative embryos at stage 12. The indicated mRNAs were each individually injected into the vegetal pole at the two-cell stage. (C) Representative embryos (bottom row) and bisections (top row) at stage 11 stained with Red-Gal and in situ hybridized for bra. (D) Representative embryos at stage 16 stained with Red-Gal and in situ hybridized for tubb2b. Arrows indicate the injected sides. Scale bars: 1 mm. |