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tbxtxenopus   

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Experiment details for tbxt

Gawantka V et al. (1998) Assay

Gene expression screening in Xenopus identifies molecular pathways, predicts gene function and provides a global view of embryonic patterning.

Gene Clone Species Stages Anatomy
tbxt.S 20e9-2 laevis NF stage 10.5 to NF stage 29 and 30 mesoderm , involuted dorsal mesoderm , involuted ventral mesoderm , tail bud

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  Fig. 3. Whole mount in situ hybridizations, sequence similarities and functional classifications. The left-most part represents the results of sequence similarity searches from 273 partially sequenced cDNAs representing unique, differentially expressed genes: clone name; accession number of best hit, P-value; name of best hit; functional classification. The order of the genes follows first digit sorting, e.g. 1, 10, 2, 20 etc. Abbreviations: o, orphan genes; m, metabolism gene; r, developmental regulator gene; r?, housekeeping regulator gene; s, structural gene; Xl, known Xenopus gene; +, GRAIL1 good coding potential; ++, GRAIL1 excellent coding potential. The P-value is the probability provided by the BLAST programme and calculated using Karlinltschul statistics, that the sequence similarity is due to randomness. For EST hits, the similarities derived from the comparison of the EST or cluster of EST sequences is given. The right-most part shows in situ hybridizations results for stages 10+, 13 and 30. Abbreviations are: a, animal view (stage 10); a, anterior view (stage 30); d, dorsal view; l, lateral view; p, posterior view; s, sagittal section; t, transverse section; v, vegetal view (stage 10); v, ventral view (stage 30). page 14

Gene Clone Species Stages Anatomy
tbxt.S 20e9-2 laevis NF stage 29 and 30 tail bud

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  Fig. 5. Tail regionalization. Whole-mount in situ hybridizations were carried out on tailbud embryos. The tail region in lateral view and vibratome sections thereof are shown. (A) Show selected marker gene expression, (N and O) show diagrams summarizing the identified domains in mid-sagittal and frontal view, respectively. Where indicated, frontal (f, posterior to the right), sagittal (s, posterior to the right), or transverse (t, dorsal to the top) vibratome sections are shown. Abbreviations: acnh, anterior chordoneural hinge; cnh, chordoneural hinge; end, endoderm; fs, forming somites; if, inner fin; mcnh, mid chordoneural hinge; of, outer fin; nec, neurenteric canal; pag, post-anal gut; pcnh, posterior chordoneural hinge; psm, presomitic mesoderm; pnc, posterior notochord; pw, posterior wall; scf, spinal cord floor; scr, spinal cord roof; so, somites; tpw, tip of posterior wall; vpw, ventral posterior wall.