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Fig. 4. Overexpression of xPTP-PESTr inhibits gastrulation. Embryos injected in the two blastomeres at two-cell stage were grown to late gastrula (Stage 13)
and treated by whole-mount in situ hybridization with the makers Sox2, brachyury (Xbra), and chordin. Embryos expressing the wild-type or the point mutant
C231S fail to close their blastopores (white double arrows) and their notochords (n) are shorter than the control (black vertical bar), indicating that gastrulation
did not occur properly. In some embryos, two notochord-like structures form around the yolk plug. The neural plate is present (Sox2) but the anterior part is
narrower (horizontal black bar) and the posterior portion splits around the yolk plug (y). |
