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Fig. 2. Expression patterns. (A) Northern blot analysis. Whole embryonic RNA isolated from various stages separated on denaturing agarose gels and probed with radiolabeled DNA for TFAP2α, TFAP2β, and TFAP2γ, as indicated. TFAP2α and TFAP2γ RNAs migrate as multiple bands. Ethidium bromide staining of 28S RNA shown as loading control. (B) Whole mount in situ hybridization. The embryonic stages are indicated to the left of the images. dors., dorsal view (anterior towards top); lat., lateral view (anterior towards right). Pronephric duct expression for TFAP2α and TFAP2β indicated by black arrowheads. In some cases (e.g., TFAP2γ, stage 17 dorsal), the deep and superficial signals yield different colors; this is an artifact of the BM-purple staining reaction. Pax8 expression in the placodal region indicated with arrowheads. |
