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Fig 4. Rai1 is critical for neural crest migration and to establish correct cartilage size. Rai1 morphants injected with 5 ng of morpholino were used for analysis. (A) in situ hybridizations of the neural crest marker, ap-2 in control (i, ii) and Rai1 morphants (iii, iv), showing an aberrant pattern of neural crest during early migration (stage 23). Arrows indicate position of the developing embryonic mouth for context. (B) in situ hybridizations of ap-2 in control (i, ii) and Rai1 morphants (iii, iv), showing an aberrant pattern of neural crest after migration (stage 30). Arrows indicate position of the developing embryonic mouth for context. (C) Cartilage labeling by Alcian Blue in control (i, ii) and Rai1 morphants (iii, iv) illustrates a decreased size of cartilage elements at stage 45. Abbreviations: eth; ethmoid, nc; nasal capsule, MK; Meckel’s, ir; infrarostral, ch; ceratohyal, ba; branchial, bh; basohyal. |