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tfap2axenopus   

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Experiment details for tfap2a

Devotta A et al. (2016) Assay

Sf3b4-depleted Xenopus embryos: A model to study the pathogenesis of craniofacial defects in Nager syndrome.

Gene Clone Species Stages Anatomy
tfap2a.L laevis NF stage 24 mandibular crest , hyoid crest , branchial crest , cranial neural crest

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  Fig. 4. Sf3b4MO2, a splice blocking morpholino interferes with neural crest formation. (A) The PCR primers to analyze sf3b4 pre-mRNA splicing are indicated (green arrows). The splice blocking morpholino (Sf3b4MO2) targets intron2/exon3 junction (red bar). (B) In Sf3b4MO2-injected embryos a shorter Sf3b4 transcript is detected (red arrow) due to loss of exon 3. (C) At neurula stage the expression of snail2 and sox10 is reduced (arrows) in Sf3b4MO2-injected embryos (30 ng), while sox2 expression is broadened (brackets). Dorsal views, anterior to top. The injected side (right) is indicated by the lineage tracer (Red-Gal). (D) The graph is a quantification of the results from four independent experiments. The number of embryos analyzed (n) is indicated on the top of each bar. (E) At tailbud stage tfap2a and sox10 expression is reduced in the pharyngeal arches (brackets). Lateral views.