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Fig. 3. E-cadherin knockdown blocks CNC migration in vivo. (A) In situ hybridization of CoMO and E-cadMO injected embryos with CNC marker twist and AP2 demonstrates that E-cadherin knockdown causes CNC migration defect. The migration can be restored by co-injection of E-cadMO resistant full-length E-cadherin. (B) Statistic of the in situ hybridization. N=number of experiments and n=number of embryos. (C) Transplanted E-cadherin knockdown CNC cells were in contrast to mbGFP injected CNC cells unable to migrate ventrally into the branchial arches. (D) Statistic of the transplantation experiment. (E) E-cadherin knockdown in CNC cells leads to branchial specific cartilage defect. Ventral view of control morpholino (CoMO) and E-cadherin morpholino injected embryo after cartilage staining with Alcian Blue at stage 44 is shown. Black dashed line indicates the reduced gill cartilage on E-cadherin depleted side of the embryo. White dashed line shows the middle line of the entire cartilage structure. * Indicates injected site and bars show the mean percentage of embryos with CNC migration defect with standard deviations. Significance is calculated according to fisher’s exact test. Scale bar: 200 μm. |
