|
|
| |
Fig. 6. The extracellular domain of E-cadherin is required for CNC migration in vivo. (A) Embryos were injected with indicated constructs and in situ hybridization with AP2 was performed at stage 25. (B) E-cadherin constructs used in the rescue experiments. S: signal peptide; EC: extracellular cadherin (repeat); TM: transmembrane domain; p120 and β-cat: p120 catenin and β-catenin binding site. (C) Statistic of the in situ hybridization. N=number of experiments and n=number of embryos. * Indicates injected site and bars show average percentage of embryos with CNC migration defect. At least three independent experiments were performed and the average percentage is shown with standard deviations. Significance is calculated according to fisher’s exact test. Scale bar: 200 μm. |
