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Fig 2. Tia1 is predominantly cytoplasmic and co-localizes with dnd1-LE-RNA at the vegetal cortex in Xenopus oocytes. (A) Temporal analysis of Tia1 expression during Xenopus oogenesis and embryogenesis. Western blot analysis of Tia1 with equivalent amounts of oocyte and embryonic extracts; stages of oogenesis and embryogenesis were as indicated. (B) Western blot analysis of Tia1 with cytoplasmic (C) and nuclear (N) fractions from staged oocytes. Igf2bp3 served as control for a cytoplasmic protein and Hnrnpab was utilized as control for a predominantly nuclear protein. (C) Co-localization analysis of endogenous Tia1 protein and vegetally localizing, microinjected Cy3-dnd1-LE in Xenopus oocytes. Tia immunostaining was performed on Cy3-dnd1-LE RNA injected stage III oocytes. Scale bars indicate 100 mm (whole oocyte) and 20 mm (magnification). |
