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Fig. 2. Whole mount in situ hybridization of gastrula stage embryos with antisense XGrg-4 and XGrg-5 probes. Dorsal views of stage 12 embryos (A, B). XGrg-5 expression along the midline is indicated by arrows (B). |
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Fig. 3. XGrg-4 and XGrg-5 expression in neurula stages. (A,B,D,E,F) Antero-dorsal views. (C) Sagittal section of a stage 16 embryo. (F) XEn-1 (Eizema et al., 1994) marks the mid-hindbrain border region. cg, cement gland, (1) stomato-hypophyseal anlage, (2) anterior neural plate, (3) prospective pros-mesencephalic boundary, (4) rim along the neural groove, (5) prospective anterior rhombencephalon. |
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Fig. 4. Expression of XGrg-4 in the ectoderm. Speckled expression of XGrg-4 in the ectoderm in stage 25 (B) and transverse section of a stage 22 embryo (A). |
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Gene |
Clone |
Species |
Stages |
Anatomy |
tle5
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laevis
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NF stage 22
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brain
,
forebrain
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midbrain
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hindbrain
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midbrain-hindbrain boundary
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spinal cord
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somite
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otic placode
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optic vesicle
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paraxial mesoderm
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pronephric mesenchyme
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lateral
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sensorial layer
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mesenchyme
,
anterior
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posterior
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Fig. 5. Localization of XGrg-4 and XGrg-5 transcripts in early tailbud stage (22). (A–C) High XGrg-4 expression at the pros-mesencephalic boundary (1), at the mes-rhombencephalic boundary (2) and in the rhombencephalon (3). (D–F) High XGrg-5 expression in the prosencephalon (1), in the anterior rhombencephalon (2) and in the posterior rhombencephalon (3). Arrows in A and D: somites. Asterisks in C and F: mesenchyme between the prospective olfactory placodes. ba, branchial arches; opv, optic vesicle; ov, otic vesicle; p, pronephros; pd, pronephric duct; sha, stomodeal-hypophyseal anlage. Embryos are viewed from lateral (A,D), dorsal (B,E) or anterior (C,F). |
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Fig. 6. Localization of XGrg-4 and XGrg-5 expression at tailbud stage 35. (A,B) XGrg-4. (C,D) XGrg-5. (1) Pros-mesencephalic boundary, (2) mes-rhombencephalic boundary. V-X: cephalic ganglia V-X. Embryos are viewed from lateral (A,C) or anterior (B,D). |