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Fig. 7. Xgrg2 and Xgrg4 inhibit XmyoD induction by MAPK signaling. Gastrula stage embryos were analysed for Xhes6 (A), Xgrg2 (B), Xgrg4 (C) and XmyoD (D–J) expression by whole-mount in situ hybridisation. (A–C) Uninjected embryos. (D–G) 1 ng flag-tagged Xgrg2, Xgrg4 or non-phosphorylatable mutant (Xgrg2AAA or Xgrg4AA) was injected with β-galactosidase mRNA (red staining) into marginal zone of a blastomere at 2-cell stage. (H–J) 1 ng of active MEK1 (MEK1S → E) mRNA was injected alone or with flag-tagged Xgrg2AAA or Xgrg4AA into a blastomere at the 2-cell stage. |
