| |
Fig. 6. Darkfield photomicrographs illustrating the localization of
xTRHR2 mRNA hybridization signal in selected regions of the brain of
white-adapted Xenopus laevis. Brain sections were dipped into Kodak
NTB-2 liquid emulsion and exposed for 1 month. A: A moderate
hybridization signal was seen over cells scattered throughout the
medial pallium (mp). B: In the medial septum (ms), many cells were
moderately labeled, although a few scattered cells showed strong
labeling. C: In the pars medialis (Apm) and pars lateralis (Apl) of the
amygdala, a high and moderate density of xTRHR2 mRNA, respectively,
was seen over several cell bodies. D: The highest concentration
of silver grains was observed in the dorsal part of the habenula (Hd),
whereas moderate labeling was seen in the ventral part (Hv). E: Control
section, consecutive to that in D, incubated with a sense riboprobe.
F: In the suprachiasmatic nucleus (SC), several cells were
strongly labeled. G: In the nucleus of the oculomotor nerve (III), a few
cells showed a moderate density of silver grains. lv, Lateral ventricle.
Scale bar 100 m. |
