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tubb2bxenopus   

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Experiment details for tubb2b

Oschwald R et al. (1991) Assay

Localization of a nervous system-specific class II beta-tubulin gene in Xenopus laevis embryos by whole-mount in situ hybridization.

Gene Clone Species Stages Anatomy
tubb2b.S laevis NF stage 9 ectoderm
tubb2b.S laevis NF stage 11.5 to NF stage 12 ectoderm , neuroectoderm , non-neural ectoderm
tubb2b.S laevis NF stage 13 pre-chordal neural plate , chordal neural plate , neural plate
tubb2b.S laevis NF stage 17 neural fold
tubb2b.S laevis NF stage 19 brain , spinal cord , cement gland primordium , eye primordium , neural tube , [+]
tubb2b.S laevis NF stage 37 and 38 brain , forebrain , midbrain , hindbrain , spinal cord , [+]
tubb2b.S laevis NF stage 43 brain , spinal cord , optic nerve , cephalic nerve , cephalic nerve

  Fig. 1. Localization of a neural-specific mRNA by whole-mount in situ hybridization. (A) Late blastula (stage 9; Nieuwkoop and Faber. 1967). The signal is found in the dorsal (O) and the ventral (V) ectoderm. AP animal pole, VP vegetal pole. (8) Mid-gastrula (stage 11 1/2, large yolk plug; lateral view) The signal can be identified on the dorsal side (O) and also in the ventral (V, presumptive epidermis) and lateral parts (YP yolk plug). (C) Late gastrula (stage 12; dorsal view). The presumptive neural plate is intensively stained (left side: cranial, right side: caudal). (DJ Early neurula (stage 13; lateral view). The signal is now found mainly in the neural plate. (E) Late neural fold stage (stage 17. dorsal view). Only the presumptive brain area and the presumptive spinal cord are stained. (F, G) Initial neural tube stage (stage 19. F dorsal view. G view on the furure head area). The signal is restricted to the presumptive brain and spinal cord (SP) zone. A/so the presumptive cement gland (CE) /s stained. (H, J) Larval stage (stage 37; H lareral view, J dorsal view). The distribution of the transcript can be found In the brain and the spinal cord. Note that also the optic nerve (OP) is stained. (KJ Advanced larva (stage 43, dorsal view). The staining is detected in the brain. the spinal cord and a/so clearly in the optic nerve (arrow).

Gene Clone Species Stages Anatomy
tubb2b.S laevis NF stage 14 neuroectoderm , pre-chordal neural plate , sensorial layer of neurectoderm

  Fig. 2 Cross section of an early neurula (stage 14, caudal part). It was incubated with antisense 35S-RNA of the clone 24-10 (f3-tubulin). The signal is observed by Epi-Polarization(Grunz. 1990c) in rhe inner layers of the medullary plate only. e epithelial layer of the neuroectoderm,s sensorial layer of the neuroectoderm, a archenteron.