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ube2d4xenopus tail 

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Experiment details for ube2d4

Jean S and Moss T (2008) Assay

A ubiquitin-conjugating enzyme, ube2d3.2, regulates xMLK2 and pronephros formation in Xenopus.

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Gene Clone Species Stages Anatomy
ube2d4.L laevis NF stage 35 and 36 tail

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  Fig. 2 Embryonic expression of ube2d3.2. (now called ube2d4) (A) A polyclonal antibody raised to amino acids 1–147 of ube2d3.2 was used to probe control embryos (uninjected) and embryos expressing FLAG-tagged ube2d3.2(-FLAG). (B) Equal quantities of total embryonic protein from the indicated stages were loaded onto an SDS-PAGE gel and probed with the polyclonal antibody raised against ube2d3.2, upper panel. A duplicate membrane was probed with pre-immune serum from the rabbit used to produce the specific ube2d3.2 antibody, middle panel (both the upper and middle panels were exposed for the same time). Tubulin was used as a loading control lower panel. ( C) Expression pattern of ube2d3.2 mRNA at stages 28 and 35. Panels show lateral views with anterior to left and the right panel is a magnification of the middle panel. AS, antisense probe. The somites (s), eye (e), otic vesicle (o), branchial arches (ba), and pronephric primordium (p) are indicated.
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