| |
Fig. 6. XSeb4R regulates the levels and expression boundaries of mRNAs encoding germ layer specification markers. XSeb4R-MO was injected into the animal hemisphere of one blastomere of two- or four-cell stage albino embryos and the latter were analyzed by WMISH at gastrula and neurula stages, using the indicated probes. The control embryo (CE) shows a ring-like expression pattern around the blastopore and the width is indicated (A). The injected embryo, oriented vegetal pole down, revealed an upward expression expansion (46%, n = 60) in the presumptive ectoderm and the width is indicated (B). Xbra analysis in neurula stage morphant embryos revealed an expanded expression in 80% of the total embryos (n = 45). Caps from CE or from embryos injected with XSeb4R-MO in the four micromeres at 4/8-cell stage were analyzed by real-time RT-PCR targeting mesodermal marker Xbra and the ectodermal gene Epi. K. XSeb4R protein knockdown induces Xbra expression and a repression of Epi. K. expression (C). Data are expressed relative to GAPDH mRNA. |
