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Figure 2. Xenopus tropicalis VegT. Whole-mount in situ hybridization analysis of tVegT mRNA expression in tropicalis oocyte and embryos. A: tVegT mRNA is localized to the vegetal half of a stage VI oocyte. The bleached animal pole is up. A lateral view of a cleared whole-mount in situ hybridization of early gastrula embryo (B) and a midsagittal section of the same whole-mount embryo(C) shows tVegT expression throughout the endoderm with stronger expression in the mesoderm. Dorsal is to the left. The brown color in the animal cap is pigment. st., embryonic stage. |