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Fig. 8. Sia/Twn promote hhex transcription by inhibiting BMP and Vent. In situ hybridization of −6.0Kb hhex:gfp transgenic embryos injected as follows: Sia/Twn-MOs (20 ng each, 4-cell dorsal), Sia/Twn-MOs (20 ng each, 4-cell dorsal) + tBR mRNA (400 pg, 8-cell dorsal–vegetal), Vent1-MO + Vent2-MO (30 ng each; 4-cell ventral), Sia/Twn-MOs (20 ng each, 4-cell dorsal) + Vent1/2-MOs (30 ng each, 8-cell dorsal–vegetal), Chordin-MO + Noggin-MO (10 ng each, 4-cell dorsal) or sia mRNA (50 pg, 8-cell ventral–vegetal). |