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Figure 7. Sfrp5 antagonizes endogenous Wnt11 signaling. (A) Reducing endogenous Wnt11 rescues Sfrp5-depleted embryos. Embryos were injected in the D1 anterior endoderm at the 32-cell stage with a wnt11-MO, sfrp5-MO, or both the wnt11-MO and the sfrp5-MO. At stage 18, the foregut epithelium was assayed by anti−β-catenin or aPKC immunostaining of bisected embryos and gene expression was examined by in situ hybridization. Injection of the wnt11-MO expanded hhex and reduced vent2 expression. Coinjection of the wnt11-MO and sfrp5-MO rescued both the epithelial integrity and the changes in gene expression observed with sfrp5-MO alone. (fgc) Foregut cavity. (B) A model of how Sfrp5 restricts both Wnt11/β-catenin and Wnt11/PCP activity to coordinate foregut specification and morphogenesis. |
