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Fig. 8. Effects of TAK1 knockdown on dorsal–ventral pattern. Embryos were injected in both dorsal blastomeres at the 4-cell stage with 20 ng of either the TAK1 MO (TAK MO) or the 5-base mispair (Mis MO); embryos were fixed at early gastrula stages (10+–10.25) and used for in situ hybridization. Dorsal-specific probes include not1 (A, B, A′, B′), otx2 (C, D), and shh (E, F). Ventrolateral probes include Xvent2 (G, H), and Xvent1 (I, J). Expression of each gene was evaluated in samples from 4 independent experiments, prepared in 2 rounds of in situ hybridization. Each set of images (for a given probe) was taken from the same round of in situ hybridization; thus, mispair and TAK1 MO embryos were processed identically, and in situs were developed for the same period of time. Images show expression patterns characteristic of at least 2/3 of embryos (TAK vs. mispair MO). |
