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Fig. 5. Manipulation of Barhl2 levels changes the number of chordin- and Xshh-expressing cells. In situ hybridization of embryos injected in either two (A-E) or one (F) dorsal blastomere with mouse Barhl2, Xbarhl2AS or human BCL2. Embryos are shown at (A) stage 10, (B,C) stage 15, (D) stage 18, (E) stage 12, (F) stage 15. (A-D) In situ hybridization using chordin as a probe. (A,B) control (a), mouse Barhl2 (b), Xbarhl2AS (c), human BCL2 (d). (C) Serial sections. (a) Scheme of a stage 14 embryo showing position of cuts. (b-i) Sections of Xbarhl2AS-injected embryo. α level: (b) control; (c) Xbarhl2AS. β level: (d) control; (e) Xbarhl2AS. γ level: (f) control; (g) Xbarhl2AS. Thinβ level sections in the prospective notochord area: (h) control; (i) Xbarhl2AS. (D) β level sections of stage 18 embryos: (a) control; (b) mouse Barhl2; (c) Xbarhl2AS. (E) In situ hybridization using Xvent2 as a probe: (a) control; (b) mouse Barhl2; (c) Xbarhl2AS. (F) In situ hybridization using Xshh as a probe (a-i). (a) Control; (b) mouse Barhl2; (c) Xbarhl2AS; (d) human BCL2. (e-h) Magnified views of a-d, respectively. (g) β level section of Xbarhl2AS-injected embryo. (h,l) In situ hybridization using gli1 as a probe. (i) In situ hybridization using gli3 as a probe. The line in e-h is shown as a scale indicator. |
