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wnt4xenopus   

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Experiment details for wnt4

Saulnier DM et al. (2002) Assay

Essential function of Wnt-4 for tubulogenesis in the Xenopus pronephric kidney.

Gene Clone Species Stages Anatomy
wnt4.L laevis NF stage 19 brain , pronephric mesenchyme , pronephric kidney , neural tube , anterior ventral blood island
wnt4.L laevis NF stage 24 brain , midbrain , hindbrain , head region , ventral blood island , [+]
wnt4.L laevis NF stage 32 brain , midbrain , hindbrain , spinal cord , endoderm , [+]

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  FIG. 1. Wnt-4 is expressed in the pronephric anlage and pronephric tubules. Wnt-4 transcripts were detected by whole-mount in situ hybridization. (A, F) Lateral views of Xenopus embryos with anterior to the left. (A) Wnt-4 expression in the pronephric anlage (arrowhead) of a stage 19 embryo. Expression is also present in the brain (b) and neural tube (nt). (B) At stage 24, Wnt-4 expression is prominently detected in the pronephric tubule anlage (arrowhead) and the ventral blood islands (bi). (C) At stage 32, Wnt-4 expression was associated with the pronephric tubules. Inset shows an enlargement of the pronephros with the three nephrostomes (arrowheads). Other regions with Wnt-4 expression include visceral arches (va), the neural tube, and discrete sections of the mid- and hindbrain (mb, hb). (D, E) Sections of stage 24 (D) and 32 (E) embryos hybridized in whole mount with Wnt-4 antisense probes. Embryos were embedded in agarose and transverse sections (30 ﰂm) were cut at the level of the developing pronephric kidney. Sections are oriented with dorsal to the top. (D) Wnt-4 expression was detected in the pronephric anlage (pa) and neural tube (nt). The inset shows an enlargement of Wnt-4 expression in the somatic layer (so) of the intermediate mesoderm. The splanchnic layer (sp) and endoderm (ed) are devoid of any expression. (E) Wnt-4 expression is associated with the developing pronephric tubules (pt) and the floor plate (fp). The inset shows a close-up view of Wnt-4 expression in the pronephric tubules. (F) A stage 32 embryo stained for Pax-2 expression to visualize the pronephric kidney with tubules (arrowheads) and duct (pd) is shown for comparative purposes. Scale bars: 600 ﰂm (A, B, C, F); 200 ﰂm (inset in C); 140 ﰂm (D, E); 40 ﰂm (insets in D, E).