| |
Fig. 5. Whole-mount in situ hybridization
shows the effect on
expression of pronephros marker
genes XTRAP-γ MO injection.
Embryos were injected with XTRAP-
γ MO (40 ng) or 5-mis-XTRAP-γ MO
(40 ng) and synthetic of β-galactosidase
mRNA (250 ng), then fixed at
stage 30/31 (A-P) and stage 22/23
(Q-T). Expression of Lim-1 was
increased in the side of embryos
injected with XTRAP-γ MO (A),
compared the non-injected side (B).
Expression of Pax-2 and Wnt-4
was inhibited by injection of XTRAP-
γ MO (E,I), compared with noninjected
side (F,J). For those embryos
fixed at stage 22/23, expression
of Pax-2 (Q) and Wnt-4 (S) in
embryos fixed at stage 22/23 was
not affected by injection of XTRAP-
γ MO or the control 5-mis-XTRAP-
γ MO (R,T). Expression of Pax-8
was not affected by XTRAP-γ MO
(M,N). The expression of Lim-1
(C,D), Pax-2 (G,H), Wnt-4 (K,L)
and Pax-8 (O,P) was unchanged in
embryos injected with 5-mis-
XTRAP-γ MO. |
