|
Gene |
Clone |
Species |
Stages |
Anatomy |
wnt4.L
|
|
laevis
|
NF stage 12
to
NF stage 13
|
gastrocoel roof plate
,
dorsal endoderm
|
wnt4.L
|
|
laevis
|
NF stage 14
to
NF stage 15
|
chordal neural plate
,
endoderm
,
liver diverticulum
,
neural plate
,
presumptive midbrain-hindbrain boundary
|
wnt4.L
|
|
laevis
|
NF stage 23
|
brain
,
midbrain
,
hindbrain
,
pronephric mesenchyme
,
cranial placode
,
[+]
|
wnt4.L
|
|
laevis
|
NF stage 27
|
brain
,
midbrain
,
hindbrain
,
lateral line system
,
foregut
,
[+]
|
wnt4.L
|
|
laevis
|
NF stage 37 and 38
|
brain
,
midbrain
,
hindbrain
,
midbrain-hindbrain boundary
,
spinal cord
,
[+]
|
|
|
Fig. 6. Localization of Xwnt-4 transcripts during early
development using whole-mount in situ hybridization. (A)
Antisense Xwnt-4 probe hybridized to a late gastrula stage
embryo (stage 12; on the left), the blastopore is pointing
down (arrowhead), and hybridized to a slightly later stage
embryo (stage 13; on the right). Xwnt-4 transcripts are
localized to a thin band of cells above the archenteron
(arrow) in the embryo on the right. (B) Antisense Xwnt-4
probe hybridized to a neurula stage embryo (stage 14/15),
shown from the side. Xwnt-4 expression occurs along the
entire anteroposterior axis where it is localized to the inner
layer of the neural ectoderm (arrow). A distinct anterior
patch of staining is denoted by the arrowhead. (C)
Antisense Xwnt-4 probe hybridized to a neurula stage
embryo (stage 14/15), shown from the top. Two anterior
patches of Xwnt-4 expression are clearly seen (arrowhead)
on the medial region of the neural plate. At this stage,
there is an absence of Xwnt-4 expression along the midline
of the neural groove (arrow). (D) Antisense XwntA probe
hybridized to an early tailbud stage embryo (stage 23).
Xwnt-4 expression in the neural tube changes by this stage,
becoming concentrated along the length of the floor plate
and restricted to the posterior end of the embryo. Three
overlapping regions of expression are seen in the border of
the prosencephalon/mesencephalon (arrow 1), the anterior
rhombencephalon (arrow 2) and the posterior
rhombencephalon (arrow 3). (E) Antisense Xwnt-4 probe
hybridized to a late tailbud stage embryo (stage 27). The
same regions of expression in the brain are denoted as in
D. (F) Antisense Xwnt-4 probe hybridized to a tadpole
stage embryo (stage 38). (G) Sense Xwnt-4 probe
hybridized to early and late tailbud stage embryos. All
embryos were rendered transparent with Murray's clear
prior to photography. The intensity of the stain varies with
respect to the length of time the embryos are incubated in
the chromogenic reaction and should not be taken as a
measure of transcript abundance when compared between
different embryos. |