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Gene/CloneSpeciesStageAnatomy ItemExperimenter
wnt4xenopus gastrocoel roof plate 

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Experiment details for wnt4

Analysis of Xwnt-4 in embryos of Xenopus laevis: a Wnt family member expressed in the brain and floor plate.

Analysis of Xwnt-4 in embryos of Xenopus laevis: a Wnt family member expressed in the brain and floor plate.

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Gene Clone Species Stages Anatomy
wnt4.L laevis NF stage 12 to NF stage 13 gastrocoel roof plate

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  Fig. 6. Localization of Xwnt-4 transcripts during early development using whole-mount in situ hybridization. (A) Antisense Xwnt-4 probe hybridized to a late gastrula stage embryo (stage 12; on the left), the blastopore is pointing down (arrowhead), and hybridized to a slightly later stage embryo (stage 13; on the right). Xwnt-4 transcripts are localized to a thin band of cells above the archenteron (arrow) in the embryo on the right. (B) Antisense Xwnt-4 probe hybridized to a neurula stage embryo (stage 14/15), shown from the side. Xwnt-4 expression occurs along the entire anteroposterior axis where it is localized to the inner layer of the neural ectoderm (arrow). A distinct anterior patch of staining is denoted by the arrowhead. (C) Antisense Xwnt-4 probe hybridized to a neurula stage embryo (stage 14/15), shown from the top. Two anterior patches of Xwnt-4 expression are clearly seen (arrowhead) on the medial region of the neural plate. At this stage, there is an absence of Xwnt-4 expression along the midline of the neural groove (arrow). (D) Antisense XwntA probe hybridized to an early tailbud stage embryo (stage 23). Xwnt-4 expression in the neural tube changes by this stage, becoming concentrated along the length of the floor plate and restricted to the posterior end of the embryo. Three overlapping regions of expression are seen in the border of the prosencephalon/mesencephalon (arrow 1), the anterior rhombencephalon (arrow 2) and the posterior rhombencephalon (arrow 3). (E) Antisense Xwnt-4 probe hybridized to a late tailbud stage embryo (stage 27). The same regions of expression in the brain are denoted as in D. (F) Antisense Xwnt-4 probe hybridized to a tadpole stage embryo (stage 38). (G) Sense Xwnt-4 probe hybridized to early and late tailbud stage embryos. All embryos were rendered transparent with Murray's clear prior to photography. The intensity of the stain varies with respect to the length of time the embryos are incubated in the chromogenic reaction and should not be taken as a measure of transcript abundance when compared between different embryos.
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