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FIGURE 2 Expression patterns of zbtb14 during early Xenopus embryogenesis. (a) Temporal expression profile of zbtb14 analyzed by semi- quantitative RT- PCR during X. laevis embryogenesis. –RT indicates whole- embryo RNA (stage 1) processed in the absence of reverse transcriptase. histone H4 was used as a loading control. (b–m) Expression pattern of zbtb14 analyzed by whole- mount in situ hybridization during the early development of X. tropicalis. Expression of zbtb14 is observed in the dorsal region (arrowheads) at the early gastrula stage (b, c and c’). Sagittal half embryos cut through the blastopore region (arrows) at the gastrula stage are shown in (c) and (d). (c’) indicates a magnified view of (c). Significant expression of zbtb14 is observed in the neural plate at early neurula stages (e–g). Cross- sections of early neurula- stage embryos (i and j), showing localized expression of zbtb14 mainly in the ectoderm (i). At late neurula stages, expression of zbtb14 is detected in the neural tube and eye region (k and l), and expression is stronger in the anterior than in the posterior region. In (d), (h), (j) and (m), hybridization with a sense probe did not show significant signals in these regions, except for non- specific staining (shown as asterisks in i, j, l, and m). Images in (b), (c), and (d) show lateral side views of gastrula- stage embryos with the dorsal lip (arrows) to the right. Images in (e), (h), and (k) show dorsal side views of embryos with posterior to the top. (f) shows the lateral side view of the embryo in (e) with posterior to the top and dorsal to the left. (g) shows the posterior side view of the embryo in (e) with dorsal to the top. Images in (l) and (m) show the frontal view with dorsal to the top. The dotted line in each panel of (i) and (j) indicates the boundary between the ectoderm and mesoderm. (n and o) X. tropicalis gastrula embryos dissected into dorsal and ventral halves were analyzed by both semi- quantitative RT- PCR (n) and qPCR (o). zbtb14 was predominantly expressed in the dorsal region at the early gastrula stage. |
