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Figure 7. Inhibition of dullard Suppresses the Expression of Early Neural Genes
mRNA of D67E (1.5 ng) or D69E (1.5 ng), control-Mo (40 ng), Dullard-Mo (40 ng), Dullard-Mo2 (20 ng), or 5-mis Mo (40 ng) was microinjected into the marginal region with β-galactosidase mRNA as a tracer. After red-gal staining, whole-mount in situ hybridization for SoxD, XSIP1, and Zic3 was performed. The percentage of embryos showing decreased expression and the total number of examined embryos are indicated in each figure. Figures for XSIP1 and Zic3 show the dorsal view. |
