|
| Gene |
Clone |
Species |
Stages |
Anatomy |
|
zeb2.L
|
|
laevis
|
NF stage 10
|
neuroectoderm
|
|
zeb2.L
|
|
laevis
|
NF stage 18
|
neural crest
,
mesoderm
,
somite
,
eye primordium
,
head
,
[+]
|
|
zeb2.L
|
|
laevis
|
NF stage 28
|
brain
,
neural crest
,
pharyngeal arch
,
eye
,
pronephric kidney
,
[+]
|
Display additional annotations [+]
|
| |
Figure 6.XZfhx1b depends on intact XMi-2β activity in early neural tissue formation. (A) Whole-mount in situ hybridization with a cRNA probe for XZfhx1b and XMi-2β at the indicated developmental stages; ba, branchial arches; ev, eye vesicle; nc, neural crest; nt, neural tube; s, somites. (B) The indicated plasmids were transfected into HEK293T cells and cells were analyzed 48 h later for the XBMP4 promoter-driven luciferase activity. β-Galactosidase-corrected luciferase values are given. Note that ZFHX1BWT represses the activity of the reporterconstruct in a dose-dependent manner, while repression by ZFHX1BAZmut proteins is significantly reduced. (C) QRT–PCR expression analysis of the indicated genes on total RNA isolated from animal caps derived from embryos injected with XZfhx1b RNA alone (200 pg/blastomere), co-injected with MOs against XMi-2β, co-injected with MOs against XMi-2β and hMi-2β RNA, co-injected with control MOs, or non-injected. Values were corrected for H4. Note that overexpression of XZfhx1b represses XBMP4 and the BMP-dependent gene XVent-1 and induces the neural gene N-CAM, while knock-down of XMi-2β alleviates XZfhx1b-dependent repression and induction of the respective genes. |
