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Figure 4. Smad10 Is Required for Formation of Organizer-Induced Tissues(A) Embryos were injected and analyzed by in situ hybridization as in Figure 2B. H, heart. (B) Two-cell embryos were injected with 5 ng morpholino into both animal poles and analyzed by RT-PCR at stage 33. WT-1 marks the kidney; NKX2.5 marks the heart. (C and D) Both animal poles of two-cell embryos were injected with morpholino and at stage 10.25 the embryos were processed for in situ hybridizations (C) or RT-PCR (D). GC, goosecoid; BU, brachyury. (E and F) Embryos were injected as in (C) and analyzed by in situ hybridization (E) with probes for XHex (stage 10.25) and endodermin (EDD, stage 27) or analyzed by RT-PCR (F) for the endodermal markers XHex (stage 10.25), Cerberus (cerb, stage 10.25), Xsox17 (both α and β isoforms, stage 10.25), and EDD (stage 27). |
