Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Search Criteria
Gene/CloneSpeciesStageAnatomy ItemExperimenter
acanxenopus cartilage tissue 

Too many results?Too few results?

Experiment details for acan

The protein kinase MLTK regulates chondrogenesis by inducing the transcription factor Sox6.

The protein kinase MLTK regulates chondrogenesis by inducing the transcription factor Sox6.

Gene Clone Species Stages Anatomy
acan.L laevis NF stage 39 cartilage tissue
acan.L laevis NF stage 41 cartilage tissue

Display additional annotations [+]
  Fig. S4. The expression patterns of chondrocyte markers in Xenopus laevis. (A-D) Whole-mount in situ hybridization for xAgc1 (A), xCrtl1 (B), xMatn1 (C) and xSox9 (D) was performed on embryos at stages 35/36, 39 and 41. OV, otic vesicle; BA, branchial arch; NT, neural tube; No, notochord; M, Meckel cartilage; CH, ceratohyal cartilage; CB, ceratobranchial cartilage; BHB, basihyobranchial cartilage; E, ethmoid cartilage; PQ, palatoquadrate cartilage.

Gene Clone Species Stages Anatomy
acan.L laevis NF stage 41 cartilage tissue

Display additional annotations [+]
  Fig. 2. xMLTK is required for chondrocyte differentiation but not for neural crest development. (A-C) xMLTK-MO or CoMO was injected unilaterally into the animal pole of two blastomeres (10 ng/cell) at the four-cell stage, and the embryos were fixed at stages 17 (A), 19 (B) and 35/36 (C). Whole-mount in situ hybridization was performed with xFoxD3 (A), xTwist (B) and xSox9 (C) probes. Brackets indicate the injected side. Frequency of specimen with the indicated phenotype (nearly equal expression) was CoMO in A, 11/11; xMLTK-MO in A, 17/19; CoMO in B, 5/6; xMLTK-MO in B, 15/15; CoMO in C, 13/13; xMLTK-MO in C, 10/12. (D) xMLTK-MO was injected into the animal pole of all blastomeres (10 ng/cell) at the four-cell stage. The heads of uninjected and injected embryos were cut out and harvested at 48, 60 and 72 hours after fertilization (corresponding to stages 35/36, 39 and 41, respectively). The expression levels of chondrocyte markers xMatn1, xAgc1 and xCrtl1 were analyzed by qRT-PCR. Representative results from two independent experiments are shown. (E) xMLTK-MO was injected unilaterally into the animal pole of two blastomeres (10 ng/cell) at the four-cell stage, and the embryos were fixed at stage 41. Whole-mount in situ hybridization was performed with xMatn1, xAgc1 and xCrtl1 probes. Arrows indicate presumptive cartilaginous elements. Frequency of specimen with the indicated phenotype (markedly reduced expression) was xMatn1, 19/21; xAgc1, 20/20; xCrtl1, 17/19.

Gene Clone Species Stages Anatomy
acan.L laevis NF stage 41 cartilage tissue

Display additional annotations [+]
  Fig. 5. xSox6 is required for chondrocyte differentiation but not for neural crest development. (A,B) xSox6-MO or CoMO was injected unilaterally into the animal pole of two blastomeres (10 ng/cell) at the four-cell stage, and the embryos were fixed at stages 17 (A) and 25 (B). Whole-mount in situ hybridization was performed with xFoxD3 (A) and xTwist (B) probes. Brackets indicate the injected side. Frequency of specimen with the indicated phenotype (nearly equal expression) was CoMO in A, 5/5; xSox6-MO in A, 18/21; CoMO in B, 5/5; xSox6-MO in B, 11/11. (C) xSox6-MO or CoMO was injected into the animal pole of all blastomeres (10 ng/cell) at the four-cell stage. Embryo heads were harvested 48 and 72 hours after fertilization (stages 35/36 and 41, respectively). The expression levels of chondrocyte markers xMatn1 and xAgc1 were analyzed by qRT-PCR. Representative results from two independent experiments are shown. (D) xSox6-MO was injected unilaterally into the animal pole of two blastomeres (10 ng/cell) at the four-cell stage, and the embryos were fixed at stage 41. Whole-mount in situ hybridization was performed with xMatn1 and xAgc1 probes. Arrows indicate presumptive cartilaginous elements. Frequency of specimen with the indicated phenotype (markedly reduced expression) was Matn1, 11/12; Agc1, 10/10.

Gene Clone Species Stages Anatomy
acan.L laevis NF stage 41 cartilage tissue

Display additional annotations [+]
  Fig. S5. The expression patterns of chondrocyte markers in Xenopus laevis. (A-C) CoMO or 5misMO was injected unilaterally into the animal pole of two blastomeres (10 ng/cell) at the four-cell stage, and the embryos were fixed at stage 41. Whole-mount in situ hybridization was performed with xMatn1 (A), xAgc1 (B) and xCrtl1 (C) probes. Frequency of specimen with the indicated phenotype (nearly equal expression) was CoMO in A, 9/11; 5misMO in A, 7/10; CoMO in B, 10/11; 5misMO in B, 11/12; CoMO in C, 9/11; xMLTK-MO in C, 12/12.