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Fig. 2. Ring canals contain actin, kelch and hts. Fragment of a 16-cell cyst, stained for actin (red) with rhodamine-phalloidin. Arrow points to the ring canal (A).
Nuclei of the same cyst counter-stained with Hoechst 33258 (B). Merged view of A and B (C). Different views of ring canals stained for actin with rhodamine phalloidin,
single ring canals (D and E). Section of the 8-cell cyst (with only six cells visible) stained with anti-kelch antibody. Arrow points to the localization
of kelch protein, nuclei (n) (F). Colocalization of actin (red) and kelch (green) in whole mount staining of a single cystocyte. Merged view (yellow). Arrow
points to the ring canal (G). Section of a fragment of ovary stained with anti-hts antibody. Hts is localized in a pattern similar to the location of ring canals (long
arrows), nucleus (n) (H). Light microscopy whole mount (A–E and G) and sections (F and H). Scale bar is equal to 10 Am in A–C, 500 nm in D– E, 7 Am in F,
25 Am in G and 10 Am in H. |
