| |
Fig. 5. Confocal microscope generated image comparisons of embryonic stage 46 hearts. Scale bar is 200 lm. The embryos were fixed in Dents fixative and
then stained with FITC-phalloidin. Images were captured at 5 nm intervals. Panels (A, E, I, M, Q, U and Y) are a compilation of 20�30 images. Panels (B,
F, J, N, R, V and Z) are single images demonstrating the maximum ventricular areas (red outline). Panels (C, G, K, O, S, W and a) are single images
demonstrating the spiral valve in the outflow tract (cyan arrows). Panels (D, H, L, P, T, X and b) are single images demonstrating the atrial septum
(magenta arrows). |
