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Fig. 1. Immunocytochemistry of
Xenopus ovary sections using
XALK4 antisera. (A) Diagram of
XALK4 protein showing the
domain regions used to design
the peptide immunogens. ECD,
extracellular domain; GS, glycineserine
rich domain (open box);
ICD, intracellular domain; STK,
serine/threonine kinase domain
(gray box); TM, transmembrane
(black box). Red lines show the
position of synthesized peptides.
Reactivity (arrows) of antisera
raised against the extracellular
domain (B), intracellular domain
(C), and serine/threonine kinase
domain (D). Spherical structure in
the cytoplasm of the previtellogenic
oocyte was visualized as
brown pigment. Bars, 50 μm. |