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Fig. 6.
Expression of ADAMTS9 during Xenopus laevis development.
(A) Analysis by RT-PCR of mRNA from egg to stage 45. Xenopus laevis stages are indicated at the top according to (Nieuwkoop and Faber, 1994) (NF) and gene names are indicated on the left. odc is a loading control. -RT is a negative control of cDNA synthesis reaction made without adding the Reverse Transcriptase enzyme and W: water (H20), is a negative control of the PCR reaction without cDNA template. (B) Analysis by WISH of ADAMTS9 expression. Developmental stages are according to (Nieuwkoop and Faber, 1994). Lateral views of embryos with anterior on the left and posterior on the right. (1) At stage 22 the expression is in the eye (e), in migrating cells (mc) and in the pronephric anlage (pn). (2, 3) At stage 25 and 26 the expression is in the eye, in migrating cells (mc) and in the midbrain hindbrain boundary (mh). (4, 5) At stage 35 and 38 the expression is in the olfactory placode (op), in the optic cup (oc), in the midbrain hindbrain boundary, in the otic vesicle (ov), in the pronephros (pn), in the pronephric duct (pd), in the branchial arches (ba), in the fin (f) and cloaca (c). (5) At stage 38 the expression is also in the lens (l), in the retinal pigment epithelium (rpe) and in the pancreas (p). |
