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Fig. S1. Staining using an adgra2 antisense and sense mRNA probe.
Comparison of the staining using an adgra2 antisense (a-d) and sense (a’-d’) probe by WMISH. Embryonic stages are indicated in each panel. Shown are lateral views with anterior to the left. Scale bars: 500 μm (a, b, a’, b’): 1 mm (c, d, c’, d’) A, anterior; D, dorsal, P, posterior, V, ventral. |
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Fig. 5. Sections of adgra2 stained Xenopus early tadpoles.
Spatial expression pattern of adgra2 is visualized by WMISH followed by cross sectioning of Xenopus embryos. a, h. Lateral views with anterior to the left at indicated stages. Dashed lines indicate the level of sections. b-g. Transversal (b-d, g) and horizontal (e, f) vibratome sections at stage 23. b. adgra2 is present in the periocular mesenchyme (POM). c, d, f. The migrating cranial neural crest cells (NCC) show adgra2 expression. e, g. adgra2 transcripts in the intersomitic arteries (IA). i-s. Horizontal (i–m) and transversal (n–s) vibratome sections at embryonic stage 25. i, j, p, r. adgra2 is visible in the intersomitic arteries (IA). k, m. At stage 25, adgra2 is detected in the branchial (BA) and hyoidal arches (HA). l. adgra2 transcripts in the posterior cranial vein (PCV). n. adgra2 is present in the periocular mesenchyme (POM). o. adgra2 is visualized in the mandibular arches (MA). q. Adgra2 transcripts are present in the second heart field (SHF). s. adgra2 is expressed in the developing myocardium (MC). A, anterior; BA, branchial arch; CG, cement gland; D, dorsal; HA, hyoidal arch; HG, hindgut; IA, intersomitic arteries; MA, mandibular arch; P, posterior; PCV, posterior cardinal vein, POM, periocular mesenchyme; S, somite; SHF, second heart field; SC, spinal cord; V, ventral. Scale bars: 1 mm (a, g). |
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adgra2 (adhesion G protein-coupled receptor A2) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 23, lateral view, anterior left, dorsal up. |
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adgra2 (adhesion G protein-coupled receptor A2) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 25, lateral view, anterior left, dorsal up. |
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adgra2 (adhesion G protein-coupled receptor A2) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 25, transverse section through the posterior pharyngeal region, dorsal up. |
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Fig. 4. Spatial expression pattern of adgra2 mRNA during Xenopus laevis embryogenesis.
Spatial expression pattern of adgra2 is visualized by WMISH. Embryonic stages are indicated in each panel. a-c. Lateral views with animal to the top. d-f. Anterior views with dorsal to the top. g-j. Lateral views with anterior to the left. f, g. The first tissue specific adgra2 expression starts at stage 23 in the periocular mesenchyme (POM), the migrating neural crest cells (NCC) and the intersomitic arteries (IA). h. At stage 25, adgra2 is expressed in the periocular mesenchyme (POM), the migrating cranial neural crest cells (NCC) and the intersomitic arteries (IA). i. At stage 30, adgra2 was detected in the branchial (BA), hyoidal (HA) and mandibular (MA) arches, the periocular mesenchyme (POM), the intersomitic arteries (IA) and the posterior cardinal vein (PCV). j. At stage 35 adrga2 transcripts were detected in the pharyngeal arches (BA, HA and MA), in the intersomitic arteries (IA), the vascular system (VS), the posterior cardinal vein (PCV) and the proctodeum (PD). a, animal; A, anterior; BA, branchial arches; BP, blastoporus; D, dorsal; HA, hyoidal arch; IA, intersomitic arteries; MA, mandibular arch; NCC, neural crest cells; P, posterior; PCV, posterior cardinal vein; PD, proctodeum; POM, periocular mesenchyme; v, vegetal; V, ventral. Scale bars: 500 μm (a–h): 1 mm (i, j). |
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Fig. 6. Sections of adgra2 stained Xenopus late tadpoles.
Spatial expression pattern of adgra2 is visualized by WMISH followed by cross sectioning of Xenopus embryos. a/j/q. Lateral views with anterior to the left at indicated stages. Dashed lines indicate the level of sections. b-g. Transversal (b, d, e, h, i) and horizontal (c, f, g) vibratome sections at stage 30. b. adgra2 is present in the periocular mesenchyme (POM). c. adgra2 transcripts are localized in the intersomitic arteries (IA). d. adgra2 is visualized in the mandibular arches (MA). e. adgra2 is expressed in the myocardium (MC) of the developing heart. f. adgra2 expression in the hyoidal (HA) and branchial arches (BA). g. Caudal section with adgra2 transcripts in the intersomitic arteries (IA). h. adgra2 is expressed in the myocardium (MC). i. adgra2 is detected in the posterior cardinal vein (PCV) and the intersomitic arteries (IA) surrounding the somites (S). k-p. Transversal vibratome sections at stage 33/34. k. adgra2 expression is detected in the periocular mesenchyme (POM). l. adgra2 expression in the intersomitic arteries (IA) and the endothelium of the posterior cardinal vein (PCV). m. adgra2 is present in the mandibular arch (MA). n-p. Transcripts of adgra2 are visualized in the heart including the second heart field (SHF), the myocardium (MC), the pericardial roof (PR), the endocard (EC) and the pericard (PC). r-u. Transversal sections at stage 35/36. r. adgra2 is visualized in the mandibular arch (MA). s-t. adgra2 expression is visible in the second heart field (SHF), myocardium (MC), the pericardial roof (PR), and the pericard (PC). r-u. adgra2 transcripts are found in the vascular system (VS) nearby the pronephric tubule (PT). A, anterior; BA, branchial arch; CG, cement gland; D, dorsal; DL, developing lens; DR, developing retina; EC, endocard; HA, hyoidal arch; HG, hindgut; IA, intersomitic arteries; MA, mandibular arch; MC, myocardium; OC, optic cup; P, posterior; PC, pericardium; PCV, posterior cardinal vein; POM, periocular mesenchyme; PR, pericardial roof; PT, pronephric tubule; S, somite; SC, spinal cord; SHF, Second heart field; V, ventral; VS, vascular system; YE, yolk endoderm. Scale bars: 1 mm (a, j, q). |
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adgra2 (adhesion G protein-coupled receptor A2) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 29 & 30, lateral view, anterior left, dorsal up. |
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adgra2 (adhesion G protein-coupled receptor A2) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 35 & 36, lateral view, anterior left, dorsal up |
