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FIG. 2. Examples of laser confocal triple immunofluorescence used to study colocalizations of AM with other pancreatic hormones in the same
slide. Each row represents a different animal: brown shark, rainbow trout, Necturus, Xenopus, Anolis, Gecko, and chicken, respectively. The first
column shows immunostaining for insulin (INS) in purple, the second contains the staining pattern for AM (green), and the third the
immunostaining for one of the monoclonal antibodies shown in red. The fourth column is a composite of the three previous ones. The
colocalization of green and red results in yellow and the colocalization of purple and green renders a white color. Colocalization of the three
colors, as can be seen in the shark, results in pink staining. |
