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Fig. 6. wt1 and foxc2 regulate glomulogenesis. (A-D′′′) Uninjected control Xenopus embryos (A-A′′′) and embryos injected with wt1-MO1 (B-B′′′), foxc2-MO (C-C′′′) or with wt1-MO1 plus foxc2-MO (D-D′′′) at stage 40 were analyzed by histological staining with Hematoxylin and Eosin (H&E, A-D), immunohistochemistry for β1-Integrin (A′-D′) or Vimentin (A′′D′′), and in situ hybridization for aplnr (A′′′-D′′′). (A′-D′) Schematics of the histology, in which the glomus is highlighted in black. The arrowhead in C′ indicates enlarged capillary loops in foxc2 morphants. c, coelom; en, endoderm; lpm, lateral plate mesoderm; lu, lung bud; no, notochord; s, somites; t, tubule. |
