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FIG. 3. The Xenopus liver (ArgL) and nonhepatic arginase
(Argl-3) have different tissue specific expression patterns. A,
the expression o f a r g l 3w as stronger in the intestinel i vthera onf stage
52-54 premetamorphic tadpoles, opposite ttoh at for argL. In addition,
argl-3 could be up-regulated by 18 h T, treatment in both tissues,
whereas ArgL could not. Five pgof total RNA from each tissue was used
per lane. The same or duplicated blots were probed with an argl-3
probe, argL or rpL8, which serveda s a loading control.B , expression of
Xenopus arginase genes in different tissues/regions of stage 60 metamorphosing
tadpoles. Ten pg of total RNA was used per lane. The
positions of the 28 and 18 S rRNA are indicated. |