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Fig. 2. Atp2a1/serca1 embryonic expression compared to myod pattern in Xenopus laevis embryos. (A). Schematic of predicted Xenopus laevis SERCA1 protein structure. The different domains of the protein are indicated: Actuator (A) domain: from aa 1 to aa 34 and from aa 122 to aa 239; Nucleotide (N) domain: from aa 360 to aa 599; Phosphorylation (P) domain: from aa 318 to aa 353 and from aa 604 to aa 753 and transmembrane (TM) domains: M1 aa 57–76; M2 aa 87–103; M3 aa 260–276; M4 aa 292–310; M5 aa760–777; M6 aa 789–806; M7 aa 832–849; M8 aa 898–914; M9 aa 931–948; M10 aa 967–984. (B). Conservation of ATP2A1–3/SERCA1–3 proteins between species. Percentage of identities and positives between Xenopus laevis SERCA1, SERCA2, SERCA3 and Homo sapiens, Mus musculus, Xenopus tropicalis SERCA1, SERCA2, SERCA3. (C–D). Atp2a1/serca1 mRNA expression from cleavage to tadpole stage in Xenopus laevis. Myod and serca1 mRNA expression were compared on stagematched sibling embryos by in situ hybridization. Whole mount (a–r) and transverse sections (m′,n′,o′,p′,q′,r′) are shown for myod (b,e,h,k,m,o,q) and serca1 (c,f,i,l,n,p,r). Blastula stage embryo (stage 9) are shown in animal pole view (a, control unstained embryo, b, c). Gastrula stage embryos are shown in vegetal view with blastopore facing top (d, control unstained embryo, e, f). Neurula stage embryos are shown in dorsal view, blastopore (yellow arrowhead) to the left (j, control unstained embryo, k–n). Tadpoles are shown in side views, head to the right. Yellow arrows in m–r indicate the level of the transverse section shown in m′–r′. n′-r′ are schematic drawings of the transverse sections. Fin, np, neural plate, nt, neural tube, so, somite, no, notochord, ec, ectoderm. Green arrows indicate hypaxial muscle cells, red arrows indicate head muscles. Scale bar: a–r 0.5 mm; m′–r′ 25 μm. |
