|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
foxi1
|
|
laevis
|
NF stage 11
|
epidermis
,
ionocyte
|
foxi1
|
|
laevis
|
NF stage 13
to
NF stage 18
|
epidermis
,
ionocyte
|
foxi1
|
|
laevis
|
NF stage 26
to
NF stage 29 and 30
|
epidermis
,
proctodeum
,
ionocyte
|
slc26a4.3
|
|
laevis
|
NF stage 13
to
NF stage 25
|
epidermis
,
ionocyte
,
non-neural ectoderm
|
slc26a4.3
|
|
laevis
|
NF stage 29 and 30
|
spinal cord
,
epidermis
,
head
,
ionocyte
|
ubp1
|
|
laevis
|
NF stage 13
|
epidermis
,
ventral
,
ionocyte
,
non-neural ectoderm
|
ubp1
|
|
laevis
|
NF stage 18
to
NF stage 29 and 30
|
epidermis
,
ionocyte
,
non-neural ectoderm
|
slc4a1
|
|
laevis
|
NF stage 15
to
NF stage 24
|
epidermis
,
ionocyte
,
non-neural ectoderm
|
slc4a1
|
|
laevis
|
NF stage 31
|
spinal cord
,
epidermis
,
ionocyte
,
anterior ventral blood island
,
retinal blood vessel
|
|
|
Fig. S3. Temporal expression of PSC genes. (A-DD) Shown is the expression of foxi1 (A-E), atp6v0a4 (F-J), pendrin (K-O), pendrin-like (P-T), ae1 (U-Y) and ubp1 (Z-DD) across different stages of embryonic development. Embryonic stage (Nieuwkoop and Faber, 1967) is marked in the lower left corner. Note that expression of foxi1 precedes any other PSC marker (compare A with F,K,P,U,Z), that expression of atp6v0a4, pendrin, pendrin-like and ubp1 are all roughly simultaneous (F,K,P,Z), and that ae1 expression does not initiate until several stages later (U). Number of cells expressing pendrin, pendrin-like or ae1 is a subset of all PSCs (for example, compare I with N,T,X; see also Figs 4 and 5 in the main text). Finally, note the ae1 expression in ventral blood islands (arrowhead in Y); a splice variant of mammalian ae1 is strongly expressed in red blood cells (Kopito and Lodish, 1985).
References
Kopito, R. R. and Lodish, H. F. (1985). Primary structure and transmembrane orientation of the murine anion exchange protein. Nature 316, 234-238.
Nieuwkoop, P. D. and Faber, J. (1967). Normal Table of Xenopus Laevis, Amsterdam: North Holland. |
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
ca2
|
|
laevis
|
NF stage 26
|
epidermis
,
ionocyte
,
non-ciliated epithelial cell
|
atp6v1b1
|
|
laevis
|
NF stage 26
|
epidermis
,
trunk region
,
ionocyte
,
non-cililated epidermal cell
|
tuba4b
|
|
laevis
|
NF stage 26
|
epidermis
,
ciliated epidermal cell
|
ca12
|
|
laevis
|
NF stage 26
|
epidermis
,
trunk region
,
ionocyte
,
non-cililated epidermal cell
|
slc26a4l
|
|
laevis
|
NF stage 26
|
epidermis
,
trunk region
,
ionocyte
,
non-ciliated epithelial cell
|
|
|
Fig. 1. Expression of ion transport genes in the X. laevis larval skin. Whole-mount in situ hybridization on stage 26 tadpoles was used to localize transcripts encoding proteins involved in ion transport, including (A,D) two subunits of the H+v-ATPase, (B,E) two isoforms of carbonic anhydrase and (C) a pendrin-like anion exchanger. Note that these genes are expressed in scattered cells that are absent from the posterior and anterior areas of skin, in contrast to the pattern of ciliated cells marked by α-tubulin expression (F). A magnified view is shown in the neighboring panels. |
|
|
|
atp6v0a4 (ATPase, H+ transporting, lysosomal V0 subunit a4 ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 26, lateral view, anterior left, dorsal up. (magnified view to right).
|