|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
c1h12orf43
|
|
laevis
|
unfertilized egg stage
|
egg
|
c1h12orf43
|
|
laevis
|
NF stage 9
|
animal hemisphere
|
c1h12orf43
|
|
laevis
|
NF stage 12
|
ectoderm
,
neuroectoderm
,
non-neural ectoderm
|
c1h12orf43
|
|
laevis
|
NF stage 15
|
neuroectoderm
,
pre-chordal neural plate
,
chordal neural plate
,
neural crest
,
neural plate
,
[+]
|
c1h12orf43
|
|
laevis
|
NF stage 20
|
pre-chordal neural plate
,
chordal neural plate
,
neural crest
,
optic vesicle
,
neural plate
,
[+]
|
c1h12orf43
|
|
laevis
|
NF stage 29 and 30
|
otic vesicle
,
brain
,
forebrain
,
midbrain
,
hindbrain
,
[+]
|
c1h12orf43
|
|
laevis
|
NF stage 35 and 36
|
otic vesicle
,
brain
,
forebrain
,
midbrain
,
hindbrain
,
[+]
|
|
|
Fig. S4. Temporal and spatial expression pattern of Custos in Xenopus embryo. (A) Xenopus Custos is expressed throughout development. The expression
level of Custos RNA was assessed by RT-PCR analysis. Ornithine Decarboxylase (ODC) was used as an internal control, RT(−) is without reverse transcriptase as
a negative control. (B) Custos is highly expressed in the anterior region of the embryo. Whole-mount in situ hybridization was performed using a Xenopus
Custos anti-sense RNA probe. No signal was detected using a Custos sense RNA probe. (C) Expression of Custos does not induce or suppress levels of activated
RhoA in HEK-293T cells; the GEF Ephexin is used as a positive control. (D) Sequence of Xenopus Custos antisense morpholino oligonucleotide (MO) is indicated
by red letters. |