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Fig. 4. Developmental expression of C3aR from gastrula to organ bud stage. (A) Whole mount in situ hybridization on a stage 10 gastrula showing expression predominantly in the marginal zone (mz); (b) blastopore. (B) Bissecting the gastrula revealed that the expression is mesodermal (m). In both (A,B) dorsal is to the right. (C) Stage 25 embryo showing expression in both the branchial segment of the neural crest (br) and in the otic vesicle (ov). Anterior is to the left, dorsal to the top. The arrow indicates early ventral blood island expression (VBI). (D) Stage 32 embryo. In addition to strong expression in cephalic structures, the presumptive liver area (li) is sur- rounded by cells expressing C3aR message. Arrow shows VBI expression. (E) Ventral view of same stage 32 embryo showing detail of VBI expression (arrow); head is to the top. (F) Isolated gut tube showing C3aR expression throughout the foregut (f) and presumptive intestine (i), (pa) pancreas. Anterior is to the left. (G) A section through the presumptive liver area (indicated in D) shows expression in lateral plate meso- derm (lpm) surrounding the anterior endoderm (ae). (H) Section through isolated gut showing staining in visceral mesoderm (vm), surrounding the presumptive intestine. |
